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目的:探讨转化生长因子(TGF)-βⅡ受体在SD大鼠胰腺发育后期的表达及意义。方法:使用RT-PCR方法分别检测交配后18.5天、新生及成年SD大鼠胰腺TGF-βⅡ受体mRNA表达;使用Western blot方法分别对上述不同时期SD大鼠胰腺TGF-βⅡ受体表达进行蛋白质水平的检测。结果:新生鼠胰腺TGF-βⅡ受体表达在核酸与蛋白质水平上均明显高于18.5天胎鼠与成年鼠胰腺,差异有显著性(P<0.05)。结论:TGF-βⅡ受体表达在新生鼠阶段有明显上调。
Objective: To investigate the expression and significance of transforming growth factor (TGF) -βII receptor in the late stage of pancreatic development in SD rats. Methods: The expression of TGF-βⅡreceptor mRNA in the pancreas of adult and adult Sprague Dawley rats was detected by RT-PCR at 18.5 days post-mating respectively. Western blot was used to detect the expression of TGF- The level of detection. Results: The expression of TGF-βⅡreceptor in the pancreas of neonatal rats was significantly higher than that of the pancreas of 18.5 days embryos and adult mice at the level of nucleic acid and protein (P <0.05). Conclusion: The expression of TGF-βⅡreceptor is obviously up-regulated in neonatal rats.