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目的观察不同日龄新生鼠脑一氧化氮 (NO)含量 ,一氧化氮合酶 (NOS)活性的变化及甲状腺激素对nNOS基因表达的调节。 方法采用丙基硫氧嘧啶 (PTU)灌注孕母鼠造成甲减模型。采用NO、NOS的生化测定法以及半定量逆转录聚合酶链反应 (RT -PCR)。 结果生化测定法显示 ,不同年龄段大鼠随着年龄的增加NO含量上升 (P <0 .0 1) ,NOS的活性上升 (P <0 .0 1) ;同一年龄段仔鼠 ,甲减组较正常组NO含量下降 (P <0 .0 1) ,NOS活性下降 (P <0 .0 1) ;RT -PCR测定显示 ,幼鼠nNOSmRNA水平随着年龄的增加而上升 ;同一年龄组 ,甲减组较正常组nNOSmRNA表达下降。 结论提示甲状腺激素对nNOSmRNA表达有上调作用 ;NO信号系统可能参与鼠脑发育阶段甲状腺激素缺乏所致的脑损害过程
Objective To observe the changes of nitric oxide (NO) content and nitric oxide synthase (NOS) activity in neonatal rats of different ages and the regulation of nNOS gene expression by thyroid hormones. Methods Propylthiouracil (PTU) was used to induce hypothyroidism in pregnant rats. Biochemical assays of NO, NOS and semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) were used. Results The biochemical assay showed that the content of NO increased with the increase of age (P <0.01) and the activity of NOS increased (P <0.01) in the same age group. In the same age group, Compared with the normal group, the content of NO decreased (P <0.01) and the activity of NOS decreased (P <0.01). The results of RT-PCR showed that the level of nNOS mRNA increased with the increase of age in the same age group Compared with normal group, nNOS mRNA expression decreased. The results suggest that thyroid hormone up-regulates the expression of nNOS mRNA; NO signaling system may be involved in the process of brain damage caused by thyroid hormone deficiency