作者用大鼠脾淋巴细胞(RSL)在体外与肾组织恒低温切片共同培养,36小时后,肾小球多阴离子(GPA)用胶性铁(CI)染色,研究不同丝裂原诱导的RSL对GPA的影响。结果:刀豆蛋白A(Con-A)诱导的RSL能引起肾小球GPA丢失,表现为肾小球CI染色力下降,其下降程度与Con-A诱导剂量有关。而Con-A诱导的RSL上清液、植物血凝素(PHA)诱导的RSL及其上清液对GPA无影响。表明:微小病变型 The authors co-cultured rat spleen lymphocytes (RSL) with cryostat sections of kidney tissue in vitro. Thirty-six hours later, glomerular polyanion (GPA) was stained with colloidal iron (CI) to study the effect of different mitogen induced RSL Impact on GPA. Results: Concanavalin A (Con-A) -induced RSL can cause the loss of glomerular GPA. The staining showed decreased glomerulus CI staining, which was related to the dose of Con-A induced. ConA-induced RSL supernatant, phytohemagglutinin (PHA) -induced RSL and its supernatant had no effect on GPA. Show: small lesions