论文部分内容阅读
目的探讨4种常见阿尔茨海默病(AD)动物模型在研究凋亡机制领域中的优势。方法采用4种常见AD模型(东莨菪碱致记忆障碍模型、鹅膏蕈酸致痴呆大鼠模型、快速老化模型、双转基因小鼠模型),利用Morris水迷宫测试动物模型认知能力;免疫印迹检测Bcl-2蛋白表达水平;TUNEL染色检测凋亡水平。结果在凋亡机制研究探讨中,快速老化小鼠(SAM)在Morris水迷宫测试、Bcl-2蛋白表达以及凋亡检测中其正常组与模型组均有显著差异(P<0.05)。结论 SAM模型在老年痴呆凋亡机制中有明显优势,为中药治疗老年痴呆的临床前研究提供指导。
Objective To investigate the advantages of four common animal models of Alzheimer’s disease (AD) in the field of apoptosis mechanisms. Methods Four common AD models (scopolamine-induced memory impairment model, gonadotrophin-induced dementia rat model, rapid aging model and double transgenic mice model) were used to assess the cognitive ability of animal models by using Morris water maze. Western blotting was used to detect Bcl -2 protein expression level; TUNEL staining apoptosis level. Results In the study of apoptosis mechanism, the rapid aging mice (SAM) showed significant difference (P <0.05) between the normal group and the model group in Morris water maze test, Bcl-2 protein expression and apoptosis detection. Conclusion The SAM model has obvious advantages in the mechanism of apoptosis in Alzheimer’s disease and provides guidance for the preclinical study of traditional Chinese medicine in treating Alzheimer’s disease.