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由于丝虫依赖蚊媒完成其发育周期,因此用一般的方法构建期特异的cDNA库材料来源困难。为解决这一难题,作者应用逆转录聚合酶链反应(RT-PCR)构建cDNA库。RT-PCR采用的引物为oligod(T)及许多线虫都具有的剪接前导序列SL_1。 SL_1是最先在自由生活线虫Caenorhab-
Because filarial insects rely on mosquito media to complete their developmental cycles, it is difficult to construct source-specific cDNA library materials in the usual way. To solve this problem, the authors used reverse transcription polymerase chain reaction (RT-PCR) to construct a cDNA library. The primers used for RT-PCR are oligod (T) and the splice leader SL_1 that many nematodes possess. SL_1 is the first live-free worm in Caenorhab-