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目的:厚朴叶中含有多种生物活性成分,提取厚朴叶中的挥发油,分析其挥发油组分,并对其进行抗菌实验研究,为开发利用这一资源提供理论依据。方法:利用水蒸气蒸馏法提取厚朴叶中的挥发油,采用气相色谱-质谱联用技术对厚朴叶挥发油的组分进行分离和鉴定,并利用正构烷烃系列物质对各组分进行定性确定,运用气相色谱面积归一化法确定各组分的相对含量,对厚朴叶挥发油选取4个细菌和1个酵母菌采用微量二倍连续梯度稀释法测定最小抑菌浓度(MIC)和平板转种法测定最小杀菌浓度(MBC)。结果:从厚朴叶挥发油中检出50种组分,鉴定出49种化合物,占全油的96.20%,厚朴叶挥发油对实验菌株均有明显地抗菌活性。结论:厚朴叶挥发油中倍半萜类物质居多,含量较高的组分为β-氧化石竹烯(32.03%),4-丙烯基苯酚(23.12%),棕榈酸(7.07%),α-亚麻酸(5.08%),桉叶油醇(4.53%)等,其挥发油对枯草芽孢杆菌和金黄色葡萄球菌的抑制和灭活效果显著。
OBJECTIVE: Magnolia bark contains a variety of bioactive components, extract the volatile oil from Magnolia officinalis leaves, analyze its volatile oil components, and conduct antibacterial experiments to provide a theoretical basis for the development and utilization of this resource. Methods: Volatile oil was extracted from leaves of Magnolia officinalis by steam distillation. The constituents of volatile oil from Magnolia officinalis were isolated and identified by gas chromatography-mass spectrometry (GC-MS), and the components were identified qualitatively by n-alkanes series , The relative content of each component was determined by gas chromatographic area normalization method. Four bacteria and one yeast were selected from the essential oil of Magnolia officinalis to determine the minimum inhibitory concentration (MIC) and plate rotation Species Determination of minimum bactericidal concentration (MBC). Results: Fifty components were identified from the essential oil of Magnolia officinalis, 49 compounds were identified, accounting for 96.20% of the whole oil. The volatile oil from Magnolia officinalis showed obvious antibacterial activity against the experimental strains. CONCLUSION: The content of sesquiterpenoids in the volatile oil of Magnolia officinalis leaves is the highest, with the highest contents of β-oxidized caryophyllene (32.03%), 4-propenylphenol (23.12%), palmitic acid Linolenic acid (5.08%), eucalyptol (4.53%) and so on, its volatile oil has significant inhibitory and inactivation effects on Bacillus subtilis and Staphylococcus aureus.