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采用ＤＮＡ分子杂交、细胞粘附试验及荧光纤维蛋白染色试验对１９６株１４个血清群ＥＰＥＣ的ｂｆｐＡ、ｅａｅＡ、和ｓｌｔ等毒力基因及其对ＨＥｐ２细胞的粘附性和致粘附脱落病变效应（ＡＥ）进行了检测。结果表明：ＥＰＥＣ中ｅａｅＡ和ｂｆｐＡ基因的携带率分别为３１１％和２３０％，所有菌株均不携带ｓｌｔ基因，２４％的菌株对ＨＥｐ２细胞表现为局灶性粘附（ＬＡ），１％的菌株为聚集性粘附（ＡＡ），未发现弥散性粘附（ＤＡ）的菌株；ＡＥ的阳性率为２４％，而且ＡＥ阳性的菌株均为ＬＡ阳性。Ⅰ类ＥＰＥＣ中ｅａｅＡ、ｂｆｐＡ、ＬＡ和ＡＥ的阳性率显著地高于Ⅱ类菌株。对ＥＰＥＣ基因和表型特征的分析表明，ｅａｅＡ和ｂｆｐＡ是ＥＰＥＣ的两个重要的毒力因子基因，ｅａｅＡ基因的表达受ｂｆｐＡ基因的影响，而且ｂｆｐＡ基因与ＬＡ和ＡＥ这两个表型特征的关系更为密切
Using DNA molecular hybridization, cell adhesion assay and fluorescent fibrin staining, the virulence genes such as bfpA, eaeA, and slt of 196 strains of 14 serogroups and their adherence to adhesions and adhesion to HEp-2 cells Lesion effects (AE) were tested. The results showed that the carrier rates of eaeA and bfpA genes in EPEC were 311% and 230% respectively, all of the strains did not carry slt gene, and 24% of strains showed focal adhesion to HEp2 cells ), 1% of the strains were aggregated adhesion (AA), and no strains of diffuse adhesion (DA) were found. The positive rate of AE was 24%, and both AE positive strains were positive for LA. The positive rates of eaeA, bfpA, LA and AE in class I EPEC were significantly higher than those in class II. Analysis of EPEC gene and phenotypic characteristics showed that eaeA and bfpA are two virulence factor genes of EPEC. The expression of eaeA gene is affected by the bfpA gene, and the bfpA gene is associated with two phenotypic characteristics of LA and AE The relationship is more closely