论文部分内容阅读
本研究旨在探讨纳秒电脉冲(nsEP)对人卵巢癌细胞铂敏感株A2780及铂耐药株C30的DNA损伤效应。将A2780和C30分别作用于场强为6kV/cm,脉宽为24ns的脉冲电场处理60s。假处理组予假脉冲处理。nsEP处理后0h、4h、8h、12h和24h采用CCK-8法检测细胞生存率;采用碱性单细胞凝胶电泳实验(彗星实验)检测细胞DNA损伤,测定彗星尾长(TL)、尾矩(TM)和Olive尾矩(OTM)的75th百分位数。结果显示随着时间增加,A2780和C30细胞的生存率和彗星形成率均先降低后增加,8h时最低(P<0.05);C30细胞的生存率高于A2780细胞(P<0.05),彗星形成率低于A2780细胞(P<0.05);C30细胞的TL、TM和OTM均低于A2780细胞(P<0.05);细胞死亡率和彗星形成率呈正相关(r=0.997,P<0.05;r=0.998,P<0.05)。结果表明,nsEP对人卵巢癌铂敏感细胞株和铂耐药细胞株的DNA损伤效应存在差异;nsEP致细胞死亡与DNA损伤相关。
This study aimed to investigate the DNA damage effects of nanosecond electrical pulses (nsEP) on platinum-sensitive A2780 and platinum-resistant C30 cells in human ovarian cancer cells. A2780 and C30 were applied to a field strength of 6kV / cm, a pulse width of 24ns pulse electric field processing 60s. The sham group was sham treated. Cell viability was determined by CCK-8 at 0h, 4h, 8h, 12h and 24h after nsEP treatment. DNA damage was measured by alkaline single-cell gel electrophoresis (comet assay), tail length (TL) and tail moment (TM) and Olive tail moment (OTM) 75th percentile. The results showed that the survival rate and comet formation rate of A2780 and C30 cells decreased at first and then increased with the increase of time, and reached the lowest at 8h (P <0.05). The survival rate of C30 cells was higher than that of A2780 cells (P <0.05) (P <0.05). The levels of TL, TM and OTM in C30 cells were lower than those in A2780 cells (P <0.05). The cell death rate was positively correlated with the rate of comet formation (r = 0.997, 0.998, P <0.05). The results showed that the DNA damage effects of nsEP on the platinum-sensitive cell lines and the platinum-resistant cell lines of human ovarian cancer were different. The cell death of nsEP was related to DNA damage.