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本文采用两种培养方法筛选耐盐变异体,结果表明,在高盐份培养基上诱导出愈伤组织后,采用直接转分化法和逐渐提高盐浓度并经低盐浓度长期继代法都可获得耐盐植株。采用前一种方法获得了冀早15、沧县红和沧县红麦三个材料的再生植株共34株。经盐池鉴定和水培鉴定其中沧县红和沧县红麦的再生植株后代中有10株的耐盐性可在R_2和R_3代遗传。采用后一种方法获得了耐2.5％NaCl的沧县红麦愈伤组织,其再生植株的R_2代有26.7％的植株其耐盐水平可达200mmolL~(-1)。而且这些耐盐细胞无性系R_2代的株高全比对照低,穗长变短,旗叶有变短的趋势,抽穗期、扬花期和有效小穗数都有不同程度的变异。
In this paper, two kinds of culture methods were used to screen salt-tolerant variants. The results showed that the direct transdifferentiation method and the gradual increase of salt concentration after prolonged generation of low-salt concentration callus induction Get salt-tolerant plants. Using the former method, 34 plants were regenerated from Hebei early 15, Cangxianhong and Cangxian red three materials. Salt pond identification and hydroponic identification of Cangxian red and Cangxian red regenerated plant progenies in 10 of the salt tolerance R_2 and R_3 generation can be inherited. Using the latter method, the callus of Cangxian county, which tolerated 2.5% NaCl, was obtained. The regenerated plants had 26.7% of R_2 generation and their salt tolerance was up to 200mmol L -1. Moreover, the plant height of R_2 generation of these salt-tolerant cell clones was lower than that of the control, the spike length became shorter, the flag leaf had a shorter trend, and the heading date, flowering period and effective spikelet number all had different degrees of variation.