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目的:初步研究非洲马铃薯水提物改善骨骼肌糖脂代谢作用机制。方法:40只雄性SD大鼠,取10只大鼠作为正常组,取30只大鼠高脂喂养1个月加一次性腹腔注射STZ造模,造模成功后分为模型组、吡格列酮组、南非马铃薯组。分组灌胃5周。每周测体质量、空腹血糖(Glu);实验结束后取血清测Glu、甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白(HDL)和低密度脂蛋白(LDL)含量;取骨骼肌测超氧化物歧化酶(SOD)、丙二醇(MDA)和细胞凋亡,测Mn SOD、GLUT4蛋白及m RNA表达情况。结果:与正常组比较,模型组大鼠Glu、TG、LDL升高(P<0.01),大鼠骨骼肌抗氧化酶SOD减少(P<0.01),线粒体抗氧化酶Mn SOD减少(P<0.01),氧化产物MDA含量增加(P<0.01),GLUT4表达受抑制(P<0.01),且TUNEL显示骨骼肌细胞凋亡增多。南非马铃薯具有改善上述异常变化的作用。结论:南非马铃薯可能通过抗氧化应激提高骨骼肌Mn SOD含量,促进GLUT4表达改善骨骼肌的糖脂代谢。
Objective: To study the mechanism of improving the glucose and lipid metabolism of skeletal muscle by using African potato water extract. Methods: Forty male Sprague-Dawley rats were randomly divided into model group, pioglitazone group, nimodipine group, South African potato group. Gavage groups for 5 weeks. Body mass and fasting blood glucose (Glu) were measured every week. Serum levels of Glu, triglyceride (TG), total cholesterol (TC), high density lipoprotein (HDL) and low density lipoprotein (LDL) Skeletal muscle were measured for superoxide dismutase (SOD), propylene glycol (MDA) and apoptosis, Mn SOD, GLUT4 protein and m RNA expression. Results: Compared with the normal group, the levels of Glu, TG and LDL in the model group were significantly increased (P <0.01), while the activities of antioxidant enzymes SOD and SOD in the skeletal muscle were decreased (P <0.01 and P <0.01) ), The content of MDA increased (P <0.01), the expression of GLUT4 was inhibited (P <0.01), and TUNEL showed that the apoptosis of skeletal muscle cells was increased. South African potato has the effect of ameliorating the above abnormal changes. Conclusion: South African potato may increase Mn SOD content of skeletal muscle by anti-oxidative stress and promote GLUT4 expression to improve glyco-lipid metabolism of skeletal muscle.