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目的:研究氧化低密度脂蛋白(Ox-LDL)对人肾小球系膜细胞植物血凝素样受体(lectin-like oxidized low-density lipoprotein receptor,LOX-1)表达。方法:不同浓度的Ox-LDL和培养的人肾小球系膜细胞共孵育,应用Real-time PCR和Western Blot方法检测Ox-LDL对人肾小球系膜细胞LOX-1表达的影响。结果:Ox-LDL剂量和时间依赖性促进人肾小球系膜细胞LOX-1mRNA和蛋白表达。Ox-LDL 40μg/mL刺激细胞0-24小时,于12小时达峰值。Ox-LDL 10、20、40、60μg/mL分别作用于细胞12小时,40μg/mL组达到峰值,为基础值的3.73倍。Ox-LDL 40μg/mL刺激细胞0-24小时,LOX-1蛋白24小时达高峰,Ox-LDL 10、20、40、60μg/mL分别作用细胞24小时,40μg/mL组细胞LOX-1蛋白达到峰值,为基础值的1.81倍。结论:Ox-LDL在一定浓度范围内剂量和时间依赖性促进人肾小球系膜细胞LOX-1表达。
AIM: To investigate the effect of Ox-LDL on the expression of LOX-1 in human glomerular mesangial cells. Methods: Ox-LDL and cultured human glomerular mesangial cells were incubated at different concentrations. Real-time PCR and Western Blot were used to detect the effect of Ox-LDL on the expression of LOX-1 in human glomerular mesangial cells. RESULTS: Ox-LDL dose-dependently increased LOX-1 mRNA and protein expression in human glomerular mesangial cells. Cells were stimulated with Ox-LDL 40 μg / mL for 0-24 hours and peaked at 12 hours. Ox-LDL 10,20,40,60μg / mL were applied to the cells for 12 hours, 40μg / mL group reached its peak, 3.73 times the base value. Ox-LDL 40μg / mL stimulated cells 0-24 hours, LOX-1 protein peaked at 24 hours, Ox-LDL 10,20,40,60μg / mL were treated cells for 24 hours, 40μg / mL group LOX-1 protein reached The peak, 1.81 times the base value. Conclusion: Ox-LDL can increase the LOX-1 expression in human glomerular mesangial cells in a dose and time dependent manner.