目的探讨SD大鼠骨髓间充质干细胞的体外分离、传代培养方法,并对培养细胞进行鉴定。方法贴壁培养法分离SD大鼠骨髓细胞,传代扩增,倒置相差显微镜进行形态学观察,流式细胞术鉴定细胞。结果原代细胞较多细胞集落,细胞规则,呈梭形、椭圆形,传代细胞多成纤维形状,第3代培养细胞表面分子CD44,CD90,CD105呈阳性表达,但不表达CD34。结论贴壁培养法培养的大鼠骨髓原代细胞可稳定表达干细胞表面的标记分子。 Objective To investigate the in vitro isolation and subculture of SD rat bone marrow mesenchymal stem cells and to identify the cultured cells. Methods The myeloid cells of SD rats were isolated by adherent culture method and passaged for amplification. The morphological changes were observed under inverted phase contrast microscope. The cells were identified by flow cytometry. Results The primary cells had many cell colonies, regular cells, spindles, oval cells and multifibrillator cells. The third generation of cultured cells showed positive expression of CD44, CD90 and CD105 but not CD34. Conclusion The primary bone marrow cells cultured in adherent culture can stably express the labeled molecules on the surface of stem cells.