论文部分内容阅读
目的研究白三烯B4(LTB4)在环氧合酶2(COX-2)抑制剂塞来昔布介导的抗癌效应中的作用。方法 MTT法检测塞来昔布、LTB4和去甲二氢愈创木酸(NDGA)对人结肠癌HT-29细胞和人前列腺癌PC-3细胞生存的影响以及LTB4对塞来昔布抗癌效应的影响。ELISA法检测塞来昔布对癌细胞中前列腺素E2(PGE2)和LTB4表达的影响。结果塞来昔布抑制HT-29和PC-3细胞的生存及LTB4表达(P<0.05或P<0.01),但仅下调HT-29细胞的PGE2表达(P<0.01)。LTB4能拮抗塞来昔布对HT-29细胞生长的抑制作用(P<0.01),NDGA明显抑制HT-29细胞的生存(P<0.01),而对PC-3细胞则没有这些作用。结论塞来昔布对HT-29和PC-3细胞的抑制效应是COX-2非依赖性的,且只有在HT-29细胞中,塞来昔布的抗癌作用主要是通过下调LTB4实现的。
AIM To investigate the role of leukotriene B4 (LTB4) in the anticancer effect mediated by celecoxib, a cyclooxygenase 2 (COX-2) inhibitor. Methods The effects of celecoxib, LTB4 and nordihydroguaiaretic acid (NDGA) on the survival of human colon cancer HT-29 cells and human prostate cancer PC-3 cells were detected by MTT assay and the effect of LTB4 on celecoxib Effect of the effect. The effect of celecoxib on the expression of prostaglandin E2 (PGE2) and LTB4 in cancer cells was detected by ELISA. Results Celecoxib inhibited the survival and LTB4 expression of HT-29 and PC-3 cells (P <0.05 or P <0.01), but only down-regulated the expression of PGE2 in HT-29 cells (P <0.01). LTB4 antagonized the inhibitory effect of celecoxib on the growth of HT-29 cells (P <0.01). NDGA significantly inhibited the survival of HT-29 cells (P <0.01), but not PC-3 cells. Conclusion The inhibitory effect of celecoxib on HT-29 and PC-3 cells is COX-2 independent, and the anticancer effect of celecoxib is mainly achieved by down-regulation of LTB4 in HT-29 cells .