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以小粒亲本博白B为轮回亲本与大粒亲本WFC杂交、连续回交,构建长粒近等基因系,发现了一个长粒对短粒显性的主效QTL。在BC2F1和BC3F2回交分离群体,粒长表现为长粒和短粒差异明显的两个组,分离比例分别符合1:1和3:1的遗传比率,表明了在BC3F2粒长主要由一个长粒显性主效QTL控制的。利用SSR标记技术,通过集团分离法和隐性个体分析法,对BC3F2进行QTL定位,结果发现控制粒长QTL(暂定名GL2(t))位于第2染色体,与RM530连锁,遗传距离为4.1cM,是个尚未报道的粒长显性主效QTL。
The QTLs for long grain to short grain were found with the parents of Bobai B as the recurrent parent and the large parent WFC by continuous backcrossing to construct the long grain near isogenic line. In the BC2F1 and BC3F2 backcross segregation populations, the grain length showed two groups with obvious difference between long grain and short grain, respectively. The segregation ratio was in accordance with the genetic ratio of 1: 1 and 3: 1, respectively, indicating that the grain length of BC3F2 mainly consisted of one long The dominant dominant QTLs are controlled by. QTL mapping of BC3F2 was carried out by group segregation and recessive individual analysis using SSR markers. The results showed that the control grain length QTL (tentative name GL2 (t)) was located on chromosome 2 and was linked to RM530 with a genetic distance of 4.1 cM, is a reported long-grain dominant QTL.