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目的探讨粒细胞集落刺激因子(G-CSF)刺激下急性白血病(AL)细胞增殖与其粒细胞集落刺激因子受体(G-CS-FR)表达的关系。方法选初诊和难治复发的急性髓性白血病(AML)患者30例,急性淋巴细胞白血病(ALL)患者20例,正常对照组20例。化疗前留取骨髓5 m l,制备骨髓单个核细胞(MNC)悬液,并分别加入不同浓度的G-CSF(5,10,15,20,25 ng/m l),培养24h后用流式细胞技术检测其DNA倍体的量。同时选用G-CSFR、CD34的单抗,采用流式细胞技术检测G-CSFR、CD34在AL和正常粒细胞的表达情况。结果骨髓MNC培养24 h后各浓度的DNA倍体量在AML随着G-CSF浓度的增加有上升趋势,在ALL和正常对照无明显变化。G-CSFR、CD34的表达率:AML:(68.59±13.99)%,(45.15±4.22)%;ALL:(1.90±0.93)%,(46.75±3.15)%;正常对照:(70.5±10.8)%,(3.15±0.22)%。AML的G-CSFR表达率与ALL比较差异有统计学意义(P<0.05),与正常对照组差异无统计学意义(P>0.05);ALL的G-CSFR表达率与正常对照比较差异有统计学意义(P<0.05)。结论G-CSF可促进AML细胞增殖,不促进ALL细胞和正常粒细胞增殖;G-CSFR主要表达于成熟及恶性粒细胞,不表达于淋巴细胞。此结果在临床治疗白血病的过程中具有指导意义。
Objective To investigate the relationship between granulocyte colony-stimulating factor receptor (G-CS-FR) expression and proliferation of acute leukemia (AL) cells stimulated by granulocyte colony-stimulating factor (G-CSF) Methods Thirty patients with acute myeloid leukemia (AML), 20 patients with acute lymphoblastic leukemia (ALL) and 20 normal controls were enrolled in this study. 5 ml of bone marrow was collected before chemotherapy to prepare bone marrow mononuclear cells (MNC) suspension, and different concentrations of G-CSF (5, 10, 15, 20, 25 ng / ml) were added respectively and cultured with flow cytometry Technology to detect the amount of DNA ploidy. At the same time, G-CSFR and CD34 monoclonal antibody were used to detect the expression of G-CSFR and CD34 in AL and normal granulocyte by flow cytometry. Results The DNA ploidy of each concentration of MNC in bone marrow MNC after 24 h culture increased with the increase of G-CSF concentration in AML, but no significant change in ALL and normal controls. The expression of G-CSFR and CD34: AML: (68.59 ± 13.99)%, (45.15 ± 4.22)%, ALL: 1.90 ± 0.93%, 46.75 ± 3.15% , (3.15 ± 0.22)%. The expression of G-CSFR in AML was significantly lower than that in ALL (P <0.05), but there was no significant difference between AML and normal controls (P> 0.05). There was statistically significant difference in G-CSFR expression between ALL and normal controls Significance (P <0.05). Conclusion G-CSF can promote the proliferation of AML cells without promoting the proliferation of ALL cells and normal granulocytes. G-CSFR is mainly expressed in mature and malignant granulocytes but not in lymphocytes. This result is instructive in the clinical treatment of leukemia.