论文部分内容阅读
探讨细胞免疫在反返流性肾病 (RN)发病中的作用。方法 :通过流式细胞仪 (FCM ) ,应用单标和双标免疫荧光法 ,利用单克隆抗体测定 12例RN患儿外周血单个核细胞 (PBMC)表面膜抗原 ,从而分析RN患者T淋巴亚群及其活化情况。结果 :T淋巴细胞亚群CD+3、CD+4 与正常组相仿 ,CD+8增高 ,CD+4 CD+8比值低于正常 (P <0 .0 1) ;T细胞CD+2 5 表达高于正常 ,T细胞亚群活化表达CD+3CD+2 5 、CD+4 CD+2 5 、CD+8CD+2 5 分别较正常组高 (P <0 .0 1) ;活性化抗原CD+ 6 9,CD+ 71 均高于正常组 (P <0 .0 1) ;相关分析显示CD+ 4 CD+ 8比值与CD+ 8、CD+ 2 5 、CD+ 3、CD+ 2 5 均存在负相关 (P <0 .0 5 )。结论 :T细胞异常活化在RN发病机制中起重要作用 ,T细胞功能紊乱与本病关系密切
To investigate the role of cellular immunity in the pathogenesis of anti-reflux nephropathy (RN). Methods: Monoclonal antibodies were used to detect the surface antigen of peripheral blood mononuclear cells (PBMCs) in 12 children with RN by flow cytometry (FCM) and single and double-labeled immunofluorescence. Groups and their activation. Results: The levels of CD + 3 and CD + 4 in T lymphocyte subsets were similar to those in normal group, while CD + 8 increased and the ratio of CD + 4 CD + 8 was lower than normal (P <0.01). CD + The expression of CD + 3 CD + 25, CD + 4 CD + 25 and CD + 8 CD + 25 in T cell subsets were significantly higher than those in normal controls (P <0.01). The activated antigen CD + 6 9 and CD + 71 were higher than those in normal group (P <0.01). Correlation analysis showed that the ratio of CD + 4 CD + 8 was negatively correlated with CD + 8, CD + 25, CD + 3 and CD + 5). Conclusion: T cell abnormal activation plays an important role in the pathogenesis of RN, and T cell dysfunction is closely related to this disease