【摘 要】
：
包含质粒载体pBI121的农杆菌菌株LBA4404被用于转化火炬松的成熟合子胚.质粒载体pBI121含有胭脂碱合成酶基因的启动子驱动的新霉素磷酸转移酶基因(nptⅡ)和花椰菜花叶病毒的3
【机 构】
：
北卡罗来纳州立大学生物技术组,北卡罗来纳大学
论文部分内容阅读
包含质粒载体pBI121的农杆菌菌株LBA4404被用于转化火炬松的成熟合子胚.质粒载体pBI121含有胭脂碱合成酶基因的启动子驱动的新霉素磷酸转移酶基因(nptⅡ)和花椰菜花叶病毒的35S启动子驱动的GUS基因.器官发生的转基因愈伤组织和转基因的再生植株已经获得,并经GUS组织化学染色、聚合酶链式反应和Southern杂交分析证实.植物激素(BA/IBA)和抗生素对转基因愈伤组织的生长和分化的影响被研究.500mg/L羧苄青霉素和2mg/L BA、0.5mg/L IBA的组合导致转基因愈伤组织的生长增加54.2%,分化增加45.7%.500mg/L Claforan和2mg/L BA、0.5mg/L IBA的组合导致转基因愈伤组织的生长增加40.8%,分化增加38.7%.高浓度的植物激素和抗生素降低了转基因愈伤组织的生长和分化.实验结果有助于建立一个高效的农杆菌介导的火炬松遗传转化系统,也有助于未来针叶树的遗传转化研究.
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