论文部分内容阅读
为深入研究太湖鲢鱼的种群遗传结构及进化过程,有效的保护和利用太湖鲢鱼种质资源,作者采用聚合酶链式反应(PCR)技术对太湖鲢鱼的mtDNA D-loop序列进行扩增,获得了大小约为500bp的扩增产物。PCR产物经纯化后克隆到pMD19-T Vector进行序列鉴定测序,得到了520bp的核苷酸片段(除去引物及部分端部序列)。用CLUSTAL X(1.83)软件进行排序比较,在30个个体中,共检测到52个变异位点,包括1个碱基缺失、36个转换位点、15个颠换位点及1个转换与颠换同时存在的位点。运用MEGA软件计算出不同个体间的遗传距离,并据此构建了NJ系统树。用DNASP软件计算出的多态位点数(S)为52、核苷酸多样性(Pi)和平均核苷酸差异数(k)分别为1.24%0.35%和6.368。研究结果表明,太湖鲢鱼的mtDNA D-loop个体序列变异程度较大,遗传多样性较为丰富。
In order to further study the population genetic structure and evolution of the Taihu silver carp, and to effectively protect and utilize the Taiyuan silver carp germplasm resources, the authors amplified the mtDNA D-loop sequence of the Taihu silver carp by polymerase chain reaction (PCR) , Obtained a size of about 500bp amplification products. After purification, the PCR product was cloned into pMD19-T vector for sequence identification and sequencing, and a 520 bp nucleotide fragment (excluding primers and partial end sequences) was obtained. A total of 52 mutations were detected in 30 individuals using CLUSTAL X (1.83) software, including 1 base deletion, 36 transition sites, 15 transversion sites and 1 transition and Transpose the same place. Using MEGA software to calculate the genetic distance between different individuals, and based on which the NJ system tree was constructed. The number of polymorphic loci (S) calculated by DNASP software was 52, and the nucleotide diversity (Pi) and mean nucleotide difference (k) were 1.24% 0.35% and 6.368 respectively. The results showed that the genetic variation of mtDNA D-loop individuals in Taihu silver carp was larger and the genetic diversity was more abundant.