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控制pH=3，首次通过同步一阶导数荧光光谱技术，有效地识别和分辨氧氟沙星的R和S对映体，同时有效地消除尿样背景的干扰。据此建立了同时识别与测定两种旋光体的同步一导数荧光光谱法，测定尿样中的对映体含量，线性范围为：0．36－31．6mg／L（R），0．36－2．89mg／L（S）和3．16－31．6mg／L（S），实际可检测下限均为0．36mg／L，回收率97％－104％，相对标准偏差小于5％。
Control of pH = 3, for the first time by synchronous first-derivative fluorescence spectroscopy, effectively identify and distinguish between the R and S enantiomers of ofloxacin, while effectively eliminating the interference of urine samples. The simultaneous one-derivative fluorescence spectroscopy was established for the simultaneous identification and determination of enantiomers. The linear range was 0.36-31.6mg / L (R), 0.36 -2.89mg / L (S) and 3.16-31.6mg / L (S). The lower limit of detection was 0.36mg / L, the recovery was 97% -104% and the relative standard deviation was less than 5%.