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目的观察过表达UCA1基因、UCA1a(CUDR)基因对人膀胱癌UM-UC-2细胞周期的影响。方法以前期实验中构建好的3组细胞[转染pcDNA-UCA1重组表达载体的UM-UC-2细胞、转染pcDNA-UCA1a(CUDR)重组表达载体的UM-UC-2细胞和转染空质粒pcDNA3.1(+)的UM-UC-2细胞(对照组)]作为材料,采用流式细胞术检测UCA1基因、UCA1a(CUDR)基因对UM-UC-2细胞周期的影响。结果与对照组相比,转染pcDNA-UCA1重组表达载体的UM-UC-2细胞、转染pcDNA-UCA1a(CUDR)重组表达载体的UM-UC-2细胞S期的细胞百分比均明显增加,G0/G1期的细胞百分比均明显减少。结论过表达UCA1基因、UCA1a(CUDR)基因均能够促进人膀胱癌UM-UC-2细胞的DNA合成,使UM-UC-2细胞增殖能力增强。
Objective To investigate the effect of UCA1 gene and UCA1a (CUDR) gene on the cell cycle of human bladder cancer cell line UM-UC-2. METHODS: Three groups of cells [UM-UC-2 cells transfected with pcDNA-UCA1 recombinant expression vector, UM-UC-2 cells transfected with pcDNA-UCA1a (CUDR) recombinant expression vector, The effect of UCA1 gene and UCA1a (CUDR) gene on the cell cycle of UM-UC-2 cells was detected by flow cytometry using the plasmid pcDNA3.1 (+) UM-UC-2 cells (control group) Results Compared with the control group, the percentage of cells in UM-UC-2 cells transfected with pcDNA-UCA1 recombinant expression vector was significantly increased in S phase of UM-UC-2 cells transfected with recombinant plasmid pcDNA-UCA1a (CUDR) G0 / G1 phase of the cell percentage were significantly reduced. Conclusion Overexpression of UCA1 gene and UCA1a (CUDR) gene can promote the DNA synthesis of human bladder cancer UM-UC-2 cells and enhance the proliferation of UM-UC-2 cells.