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本文主要研究了2个耐热性不同的二倍体小麦基因型中热击蛋白(HSP)基因的表达。M_3(耐热)与M_9(热敏感)品种在耐热性方面的显著差异通过以下几个指标来决定:热击胁迫后的恢复生长情况;叶绿素的含量;以及热致死处理前12h的热锻炼下的2,3,5—三苯基四唑氯化物的还原反应。结果表明,只有致死处理前2h的热锻炼下,电镜观察的M_3的细胞超微结构较M_9表现出较稳定的耐热性,而离体翻译的热击蛋白质双向电泳分析并未观测到2个品种间的HSP分布的数量差异。用HSP cDNA16.9、26和70探针做Northern分析,表明2h热锻炼期间M_3较M_9有较高的HSP mRNA稳态水平。从而说明了小麦耐热性水平的提高与热胁迫前2h热击基因表达的水平有关。
This paper mainly studied the expression of heat shock protein (HSP) gene in two diploid wheat genotypes with different heat tolerance. The significant differences in heat tolerance between M_3 (heat-tolerant) and M_9 (heat-sensitive) cultivars were determined by the following indicators: recovery growth after heat shock stress; chlorophyll content; and thermal exercise 12 h before heat-killed Reduction of 2,3,5-triphenyltetrazolium chloride. The results showed that the cell ultrastructure of M 3 observed by electron microscopy showed more stable heat resistance than that of M 9 only under hot exercise 2 h before lethal treatment, while the two-dimensional electrophoresis analysis of ex vivo hot-swapping protein did not observe 2 Differences in the number of HSPs among varieties. Northern analysis using the HSP cDNA 16.9, 26 and 70 probes showed that M_3 had higher HSP mRNA homeostasis than M_9 during the 2h thermal exercise. Which indicated that the increase of heat tolerance level in wheat was related to the level of heat shock gene expression 2 h before heat stress.