论文部分内容阅读
目的:建立检测一种人血浆中阿米替林液相色谱-串联质谱(LC-MS/MS)的方法。方法:加入地西泮为内标,血浆中的药物用乙醚液-液萃取去蛋白。采用Agilent Zorbax SB-C18(2.1 mm×150 mm,5μm)柱,流动相为甲醇-0.1%甲酸溶液(70∶30,v/v)。采用电喷雾离子源(ESI),正离子检测,多反应监测(multiple reaction monitoring,MRM)方式进行定量分析阿米替林,监测碎片离子峰分别为m/z233,91(阿米替林)和m/z257,222(内标)。结果:血浆中阿米替林检测方法的线性范围为2.08～1040 ng.mL-1,最低检测限为0.52 ng.mL-1,平均萃取回收率在67.5%～75.5%之间,日内和日间精密度RSD都小于7%。结论:本方法简单、灵敏,可用于临床毒物分析和药物动力学研究。
Objective: To establish a method for the determination of amitriptyline in human plasma by liquid chromatography-tandem mass spectrometry (LC-MS / MS). Methods: With diazepam as internal standard, the plasma drug was deproteinized by ether-liquid extraction. An Agilent Zorbax SB-C18 (2.1 mm × 150 mm, 5 μm) column was used with a mobile phase of methanol-0.1% formic acid (70:30, v / v). Amitriptyline was quantified by electrospray ionization (ESI), positive ion detection and multiple reaction monitoring (MRM). The ion peaks monitored were m / z 233 and 91 (amitriptyline) and m / z 257, 222 (internal standard). Results: The linear range of amitriptyline in plasma was 2.08-1040 ng.mL-1, the lowest detection limit was 0.52 ng.mL-1, the average recovery was between 67.5% -75.5%. The days and days Inter-precision RSD is less than 7%. Conclusion: The method is simple and sensitive and can be used for clinical analysis of toxicology and pharmacokinetics.