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为合理利用丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)资源,揭示AM真菌对金橘根围土壤酶活性和植株生长影响的机制,以喀斯特非岩溶区酸性土(AS)和岩溶区石灰土(LS)为基质进行盆栽实验,研究分别接种丛枝菌根真菌AMF的摩西球囊霉(Glomus mosseae,G.m)和幼套球囊霉(Glomus etunicatum,G.e)对金橘幼苗根围土壤酶活性的影响。结果表明:相同类型土壤中分别接种G.m和G.e后,无论何种土壤,G.e处理金橘根围菌根侵染率各指标F、m、v均显著高于G.m处理(P≤0.05),其中酸性土中接种G.e处理的最高;此外,分别接种G.e和G.m后,金橘根围土壤蛋白酶、脲酶、磷酸酶和蔗糖酶活性与土壤有机碳、有机质含量及植株生物量均比其相应的不接种对照显著增高。蛋白酶和脲酶活性变化趋势为:(LS+G.e)>(LS+G.m)>(AS+G.e)>(AS+G.m),蔗糖酶和磷酸酶活性变化则为:(AS+G.e)>(AS+G.m)>(LS+G.e)>(LS+G.m),差异均达显著水平(P≤0.05)。G.e处理金橘根系土壤蛋白酶活性增幅最大,脲酶第二,磷酸酶最小。土壤有机质和有机碳含量变化均有(AS+G.e)>(LS+G.e)>(LS+G.m)>(AS+G.m)的变化趋势,G.e处理均显著高于G.m处理(P≤0.05)。不论酸性土或石灰土,金橘干物质积累表现为接种G.e处理显著高于G.m处理,表现出与AM菌根侵染率相似的变化趋势。可见,AMF可通过增强金橘根围土壤酶活性,影响土壤中有效养分的转化和土壤肥力,最终促进金橘生长。G.e为筛选出的能与喀斯特地区石灰土和酸性土中生长的金橘共生良好的优势菌种,具有较好的运用潜力以克服喀斯特地区土壤肥力低而导致的作物收成欠佳的难题。
In order to make rational use of arbuscular mycorrhizal fungi (AMF) resources, the mechanism of arbuscular mycorrhizal fungi (AMF) on the soil enzyme activities and plant growth in kumquat rhizosphere was revealed. Based on karst karst acidic soil (AS) ) Was used as substrate to study the effects of Glomus mosseae (Gm) and Glomus etunicatum (Ge) on soil enzyme activities in the rhizosphere soil of Kumquat . The results showed that after inoculating Gm and Ge into the same soil respectively, the F, m and v of root inoculation were significantly higher than those of Gm (P≤0.05) In addition, after Ge and Gm were inoculated respectively, the activities of soil protease, urease, phosphatase and sucrase and soil organic carbon, organic matter content and plant biomass were significantly higher than those of corresponding non-inoculated control . (LS + Ge)> (LS + Gm)> (AS + Ge)> (AS + Gm). The changes of sucrase and phosphatase activities were as follows: (AS + Ge)> + Gm)> (LS + Ge)> (LS + Gm), the differences were significant (P≤0.05). G.e treatment kumquat roots maximum increase in soil protease activity, urease second, minimum phosphatase. The changes of soil organic matter and organic carbon content showed the trend of (AS + G.e)> (LS + G.e)> (LS + G.m)> (AS + G.m), and the G.e treatment was significantly higher than that of G.m treatment (P≤0.05). The accumulation of dry matter of kumquat in both soil and lime soil was significantly higher than that of G.m and showed a similar trend as AM mycorrhizal infection. It can be seen that AMF can promote the growth of kumquat by enhancing soil enzyme activities in the rhizosphere of kumquat, affecting the conversion of available nutrients in the soil and soil fertility. G.e is a dominant species that can be selected and symbiosed with kumquat grown in karst areas and kumquats growing in acid soils, and has good application potential to overcome the problem of poor crop yields due to low soil fertility in karst areas.