论文部分内容阅读
目的:探讨精子DNA损伤、精子核蛋白组型转换与顶体酶活性及精液参数的相关性。方法:收集535例精液标本,采用精子染色质扩散(sperm chromatin dispersion,SCD)检测精子DNA损伤,并与精子核蛋白组型转换、顶体酶活性和WHO手册(第4版)精液参数进行相关性分析。结果:精子DNA损伤与精子核蛋白组型转换、顶体酶活性、精子浓度及前向运动精子这些指标之间比较均具有显著性差异(P<0.01);精子DNA损伤与年龄、核蛋白组型转换、精子浓度和D级精子比例之间呈显著正相关(P<0.01或P<0.05),而与顶体酶活性呈显著负相关(P<0.001),多元逐步回归分析显示年龄、精子浓度、D级精子比例、核蛋白组型转换及顶体酶活性是5个独立的相关变量。精子核蛋白组型转换、顶体酶活性、精子密度和前向运动精子这4个指标的异常率在精子DNA异常组(DFI≥30%)中均显著的高于正常组(DFI<30%)。结论:精子DNA损伤与精子核蛋白组型转换、顶体酶活性及WHO(第4版)精液各参数之间存在密切的联系,可能是评价精子质量的另一项重要的指标。
Objective: To investigate the correlation between sperm DNA damage, sperm nucleoprotein conversion and acrosin activity and sperm parameters. Methods: 535 cases of semen samples, using the sperm chromatin dispersion (sperm chromatin dispersion, SCD) detecting sperm DNA damage, and sperm nucleoprotein conversion, acrosomal enzyme activity and the WHO Handbook (4th Edition) parameters related to sperm Sexual analysis. Results: Sperm DNA damage and sperm nucleoprotein conversion, acrosomal enzyme activity, and the concentration of sperm forward motility index comparison between the differences were significant (P <0.01); sperm DNA damage associated with age, nucleoprotein (P <0.01 or P <0.05), but negatively correlated with acrosin activity (P <0.001). Multivariate stepwise regression analysis showed that age, sperm Concentration, D grade sperm ratio, nuclear protein type conversion and acrosin activity are five independent variables. The abnormal rate of sperm nucleoprotein conversion, acrosin activity, sperm density and forward motile sperm in sperm DNA abnormal group (DFI≥30%) were significantly higher than those in normal group (DFI <30% ). CONCLUSIONS: There is a close relationship between sperm DNA damage and sperm nucleoprotein type switching, acrosin activity and semen parameters of WHO (4th edition), which may be another important index for evaluating sperm quality.