用柱层析法测定陕西沙棘种籽油和果肉油中胡萝卜素的含量。吸附剂为草酸钠∶滑石粉(1∶1);洗脱剂为石油醚∶丙酮(9∶1)。称取种籽油1g(或果肉油0.25g),用洗脱剂溶解,定容至10ml棕色容量瓶中,取样品液1ml上柱进行洗脱,收集洗脱液50ml。以β-胡萝卜素为对照品,在440nm处测定并计算含量,线性范围为0.4～2.5μg/ml,相关系数r=0.9999。回收率为96.10±1.09%。此方法简便,色层分 Determination of Carotene Content in Shaanxi Seabuckthorn Seed Oil and Fruit Oil by Column Chromatography. The adsorbent was sodium oxalate:talc (1:1); the eluent was petroleum ether:acetone (9:1). Weigh 1g of seed oil (or 0.25g of fruit oil), dissolve with eluent, dilute to a 10ml brown volumetric flask, and take 1ml of sample solution on the column to elute and collect 50ml of eluent. The content of β-carotene was measured and calculated at 440 nm with a linear range of 0.4-2.5 μg/ml and a correlation coefficient of r=0.9999. The recovery rate was 96.10±1.09%. This method is simple and color layered