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目的:对壮药排钱草根进行抗肝纤维化活性成分研究。方法:通过多种色谱技术结合光谱学手段对排钱草根中的化学成分进行分离与结构鉴定。利用乙醛诱导的小鼠肝星状细胞(HSC-T6)增殖模型对所得到的化合物进行抗肝纤维化活性评价。结果:从排钱草根中分离鉴定了10个化合物,分别为:3,5,2’,4’-tetrahydroxy-2″,2″-dimethylpyrano-[5″,6″,7,8]-flavanone(1)、异柠檬酚(2)、citflavanone(3)、8-prenylated 5,7,3’,4’-tetrahydroxy flavanone(4)、pulchelstyrene A(5)、pulchelstyrene B(6)、pulchelstyrene D(7)、3-indolcarbaldehyde(8)、3-methoxyindole(9)、对羟基苯甲酸(10)。结论:其中,化合物1~4、8~10为首次从该植物中分离得到。体外活性评价结果显示化合物2、4~6对乙醛诱导的小鼠肝星状细胞增殖具有一定的抑制作用。
Objective: To study the anti-hepatic fibrosis activity of the drug-releasing grass roots. Methods: The chemical constituents of the root of Paecilomyces tenuardis were isolated and identified by a variety of chromatographic techniques combined with spectroscopic methods. The resulting compounds were evaluated for anti-hepatic fibrosis activity using acetaldehyde-induced mouse hepatic stellate cell (HSC-T6) proliferation model. Results: Ten compounds were isolated and identified from the roots of perennial cashmere, respectively as 3,5,2 ’, 4’-tetrahydroxy-2 “, 2” -dimethylpyrano- [5 “, 6”, 7,8] -flavanone (1), citric acid (3), 8-prenylated 5,7,3 ’, 4’-tetrahydroxyflavanone (4), pulchelstyrene A (5), pulchelstyrene B 7), 3-indolcarbaldehyde (8), 3-methoxyindole (9), p-hydroxybenzoic acid (10). Conclusion: Among them, compounds 1 ~ 4 and 8 ~ 10 were isolated from this plant for the first time. In vitro activity evaluation showed that compounds 2,4 and 6 could inhibit the proliferation of mouse hepatic stellate cells induced by acetaldehyde.