论文部分内容阅读
SBP基因家族是植物中一类特有的转录因子家族,广泛参与植物的生长发育以及各种生理生化反应的信号传导。为了揭示番茄SBP基因家族的功能,本研究利用全基因组信息鉴定番茄SBP转录因子家族,分析其结构特征,系统发育关系以及表达模式。结果表明,番茄全基因组共编码17个SBP转录因子成员,分布在8条染色体上;系统发育关系表明SBP基因家族的结构特征变异发生在番茄、拟南芥和水稻分化之前;且它们在番茄、拟南芥和水稻中按照物种特异性的方式进行了扩张。不同芯片分析发现,SlSBP03基因在番茄叶片、子叶和下胚轴中表达量较高,SlSBP13基因果肉中表达量较高,这两个基因在果皮和根中表达量都较低;在大多数非生物胁迫处理条件下,SlSBP11、SlSBP13和SlSBP16的表达量均较低;而SlSBP01基因在干旱和热处理条件下有较高的表达量。
The SBP gene family is a family of unique transcription factors in plants that are widely involved in plant growth and development as well as signal transduction of various physiological and biochemical reactions. In order to elucidate the function of tomato SBP gene family, we used genome-wide information to identify the tomato SBP transcription factor family and analyzed its structural characteristics, phylogenetic relationships and expression patterns. The results showed that the genome of tomato encodes 17 members of SBP transcription factor, which are distributed on 8 chromosomes. The phylogenetic relationship indicates that the structural variation of SBP gene family occurs before tomato, Arabidopsis and rice differentiation. Arabidopsis and rice have been expanded in a species-specific manner. Different chip analysis showed that SlSBP03 gene was highly expressed in tomato leaves, cotyledons and hypocotyls, and the expression level of SlSBP13 gene was higher in the flesh and both were lower in the pericarp and root. Under the conditions of biotic stress, the expression levels of SlSBP11, SlSBP13 and SlSBP16 were low, while the expression level of SlSBP01 gene was higher under drought and heat treatment conditions.