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目的调查漳平市2所农村幼儿园急性出血性结膜炎(AHC)的疫情与病原,为防控提供依据。方法调查疫情,采集10例病人眼拭子标本,用人横纹肌细胞进行病毒分离,实时荧光RT-PCR法进行检测。结果本次疫情共发生AHC 15例,罹患率为4.1%。患者均有畏光流泪、眼结膜充血、刺痛、分泌物增多等症状,病程平均5d,无并发症;均居家隔离并用眼药水等消炎对症治疗后痊愈。对10份标本分泌物直接提取病毒核酸检测,4份肠道病毒通用、柯萨奇病毒A组24型变种(CVA_(24v))均阳性,人肠道病毒70型(HEV_(70))均阴性。经敏感细胞培养分离到CVA_(24v)9株。结论该次AHC疫情由CVA_(24v)引起,应加强校园AHC防治的健康教育,加强主动监测和预警。
Objective To investigate the outbreaks and pathogens of acute hemorrhagic conjunctivitis (AHC) in 2 rural kindergartens in Zhangping City, and to provide basis for prevention and control. Methods The epidemic situation was investigated. The eye swab samples from 10 patients were collected and isolated from human striated muscle cells by real-time fluorescent RT-PCR. Results A total of 15 cases of AHC were observed in this outbreak and the attack rate was 4.1%. Patients had photophobia, conjunctival hyperemia, tingling, increased secretions and other symptoms, duration of an average of 5d, no complications; all home isolation and eye drops with anti-inflammatory symptomatic treatment recovered. The virus nucleic acid was directly extracted from 10 specimens, 4 of which were common, 24 of Coxsackievirus A (CVA 24v) were positive, and all of human enterovirus 70 (HEV 70) negative. Nine strains of CVA_ (24v) were isolated by sensitive cell culture. Conclusion The outbreak of AHC was caused by CVA_ (24v). Health education should be strengthened to prevent and control AHC on campus, and active surveillance and early warning should be strengthened.