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目的:研究三七对大鼠肝组织内细胞色素P450(cytochrome P450,CYP)和谷胱甘肽转移酶(glutathioneS-trans-ferase,GST)基因表达的影响。方法:雄性SD大鼠,三七2,4 g.kg-1,每日1次连续灌胃14 d,用逆转录-实时定量PCR法检测肝组织内的CYP1A1,CYP1A2,CYP2B1,CYP2E1,CYP3A1,CYP4A1基因和GST-m1,GST-pi基因的表达情况。结果:三七不影响肝组织内CYP1A1,CYP1A2,CYP2E1,CYP3A1,GSTm1和GST-pi基因的表达,2,4 g.kg-1的三七可明显抑制CYP2B1基因(0.48倍,P<0.05和0.61倍,P<0.05)和CYP4A1基因(0.69倍和0.51倍)的表达。结论:三七可选择性地抑制大鼠肝组织内CYP2B1和CYP4A1的基因表达,这可能是三七的作用机制之一。三七对CYP1A1,CYP1A2,CYP2E1和CYP3A1的基因表达没有影响,提示三七与以上主要药物代谢酶代谢的药物合用时在肝内不会产生代谢性药物相互作用。
Objective: To investigate the effect of notoginseng on the expression of cytochrome P450 (CYP) and glutathione S-trans-ferase (GST) in rat liver tissue. Methods: Male Sprague-Dawley rats were treated with 2,4,7-g-1, 2,4-g.kg-1 once daily for 14 days. The levels of CYP1A1, CYP1A2, CYP2B1, CYP2E1 and CYP3A1 in liver tissues were detected by reverse transcription-real- , CYP4A1 gene and GST-m1, GST-pi gene expression. Results: Panax notoginseng did not affect the expression of CYP1A1, CYP1A2, CYP2E1, CYP3A1, GSTm1 and GST-pi in liver tissue. 0.61-fold, P <0.05) and CYP4A1 gene (0.69-fold and 0.51-fold). Conclusion: Panax notoginseng can selectively inhibit the gene expression of CYP2B1 and CYP4A1 in rat liver tissue, which may be one of the mechanisms of Panax notoginseng. Notoginsenoside has no effect on the gene expression of CYP1A1, CYP1A2, CYP2E1 and CYP3A1, suggesting that notoginsenoside does not produce metabolic drug interactions in the liver when combined with the above drugs metabolized by major drug metabolizing enzymes.