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HYW-8是本实验室自行分离的一株对鳞翅目害虫具有高毒力的苏云金芽孢杆菌,PCR-RFLP鉴定含有cry1Ab、cry1E基因和一种新的cry2A基因,根据cry2A全长基因序列设计特异引物,获得全长基因.测序结果表明,该基因开放阅读框为1 896 bp,编码632个氨基酸,相对分子质量(Mr)是70.774×103,等电点pI=7.79,与已知的cry2Ah2基因编码的蛋白的同源性最高为98%.该基因序列已在GenBank中登记注册,登录号为GU073380,并被国际Bt杀虫晶体蛋白基因命名委员会正式命名为cry2Ah3.SDS-PAGE分析,HYW-8菌株表达130×103大小蛋白,未见70×103大小蛋白.
HYW-8 is a self-isolated Bacillus thuringiensis strain isolated from our laboratory, and identified by PCR-RFLP, which contains cry1Ab and cry1E genes and a novel cry2A gene. According to the cry2A full-length gene sequence design Specific primers to obtain the full-length gene.The sequencing results showed that the open reading frame of the gene was 1 896 bp, encoding 632 amino acids, the relative molecular mass (Mr) was 70.774 × 103, the isoelectric point pI was 7.79, which was similar to the known cry2Ah2 The sequence of the gene was registered in GenBank with the accession number of GU073380 and was officially named cry2Ah3 by the International Bt Insecticidal Crystal Protein Gene Nomenclature Committee.DS-PAGE analysis showed that HYW -8 strain expressed 130 × 103 protein, no 70 × 103 protein.