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目的:通过裸鼠体内实验探讨GRIM-19对子宫颈癌细胞血管生成的影响。方法:采用western blot检测GRIM-19和VEGF的mRNA和蛋白水平表达情况。将HeLa/Control、HeLa/GRIM-19接种到裸鼠皮下检测荷瘤情况,利用免疫组化法计数微血管密度。结果:HeLa/GRIM-19细胞接种肿瘤生长速度明显减慢(P<0.01)。移植瘤蛋白检测发现GRIM-19表达升高,而VEGF的表达降低。HeLa/Control移植瘤的微血管密度是72±9.3/mm2,而HeLa/GRIM-19的微血管密度为32±4.7/mm2,两者差异有统计学意义(P<0.05)。结论:GRIM-19表达可以抑制子宫颈癌细胞的微血管生成,可能是子宫颈癌治疗的新靶点。
OBJECTIVE: To investigate the effect of GRIM-19 on the angiogenesis of cervical cancer cells in nude mice in vivo. Methods: Western blot was used to detect the mRNA and protein expression of GRIM-19 and VEGF. HeLa / Control, HeLa / GRIM-19 were inoculated into nude mice subcutaneously to detect the tumor-bearing condition, and the microvessel density was counted by immunohistochemistry. Results: The tumor growth rate of HeLa / GRIM-19 cells was significantly slowed down (P <0.01). Transplantation tumor protein test found GRIM-19 expression increased, while the expression of VEGF decreased. The microvessel density of HeLa / Control xenografts was 72 ± 9.3 / mm2, while the microvessel density of HeLa / GRIM-19 was 32 ± 4.7 / mm2, with statistical significance (P <0.05). Conclusion: The expression of GRIM-19 can inhibit the micro-angiogenesis of cervical cancer cells and may be a new target for the treatment of cervical cancer.