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目的:探讨幽门螺杆菌(Hp)感染与儿童哮喘发病的关系以及对免疫功能的影响。方法:随机选取2013年1月~2016年4月我院收治的哮喘儿童95例,定义为哮喘组,同时选择来我院做儿保的健康儿童100例定义为对照组,测定两组患儿Hp感染情况;采集哮喘组儿童的静脉血,对比Hp阳性哮喘患儿与Hp阴性哮喘患儿外周血T淋巴细胞亚群辅助性T细胞1(Th1)、辅助性T细胞2(Th2)、Th1/Th2、辅助性T细胞17(Th17)、调节性T细胞(Treg)、Th17/Treg分布情况和血清细胞因子白细胞介素1-β(IL1-β)、白细胞介素-2(IL-2)、γ干扰素(IFN-γ)、白细胞介素-4(IL-4)、白细胞介素5(IL-5)、白细胞介素-17(IL-17)、白细胞介素-25(IL-25)含量。结果:哮喘组儿童Hp阳性率为36.84%,显著高于对照组的16.00%(P<0.05);哮喘组发作期儿童Hp阳性率为54.10%,显著高于哮喘组缓解期儿童的5.88%(P<0.05);Hp阳性哮喘患儿的Th1细胞、Th1/Th2比例显著低于Hp阴性哮喘患儿(P<0.05),Th2细胞、Th17细胞、Th17/Treg比例均显著高于Hp阴性哮喘患儿(P<0.05);Hp阳性哮喘患儿IL1-β、IL-4、IL-5、IL-17、IL-25含量显著高于Hp阴性哮喘患儿(P<0.05),IL-2、IFN-γ含量显著低于Hp阴性哮喘患儿(P<0.05)。结论:哮喘儿童Hp感染率高于健康儿童,随着病情的进展,Hp阳性率升高,Hp感染能够显著造成患儿免疫系统功能紊乱,导致哮喘发生或加重,临床应给予重视。
Objective: To investigate the relationship between Helicobacter pylori (Hp) infection and childhood asthma and its influence on immune function. Methods: A total of 95 asthmatic children admitted to our hospital from January 2013 to April 2016 were randomly selected and defined as asthma group. Meanwhile, 100 healthy children selected as our child care center in our hospital were defined as the control group. Two groups of children Hp infection. The venous blood of children with asthma was collected to compare the levels of T helper 1 (T helper 1), T helper 2 (Th2) and Th1 in children with Hp-negative asthma and Hp- / Th2, Th17, Treg and Th17 / Treg and the levels of IL-1, IL-2, IFN-γ, IL-4, IL-5, IL-17, IL- -25) content. Results: The positive rate of Hp in asthmatic children was 36.84%, which was significantly higher than that in control group (16.00%, P <0.05). The positive rate of Hp in asthmatic children was 54.10%, significantly higher than that in asthmatic children (5.88% P <0.05). The Th1 / Th2 ratio of Th1 cells in Hp-positive asthmatic children was significantly lower than that in Hp-negative asthmatic children (P <0.05), and the Th2 cells, Th17 cells and Th17 / Treg ratio were significantly higher than those in Hp-negative asthmatic patients IL-2, IL-5, IL-17 and IL-25 in Hp-positive asthmatic children were significantly higher than those in Hp-negative asthmatic children (P <0.05) IFN-γ content was significantly lower than Hp-negative asthmatic children (P <0.05). Conclusion: The prevalence of Hp infection in children with asthma is higher than that in healthy children. As the disease progresses, the positive rate of Hp increases. Hp infection can cause dysfunction of immune system in children with asthma, leading to asthma exacerbation.