作者报道了RPMI培养液、全血(WB)或去白细胞的血液(LDB)中较高浓度的抗坏血酸(AA)对人类免疫缺陷症病毒(HIV)的体外灭活的作用。 首先进行无细胞(CF)HIV滴定,以指示细胞的合胞体形成单位(sfu)表示病毒含量。在RPMI培养液、WB及LDB中均加入终浓度为0.25×10~5sfu的CFHIV。其中RPMI(4份)中AA浓度分别为62.5、125、250和500μg/ml,WB及LDB中AA浓度为500μg/ml。混合液经4C保温3小时后,将RPMI、WB The authors reported the effect of higher concentrations of ascorbic acid (AA) in RPMI broth, whole blood (WB) or leukocyte-depleted blood (LDB) on the in vitro inactivation of human immunodeficiency virus (HIV). Cell-free (CF) HIV titrations were first performed to indicate that the cells’ syncytium formation units (sfu) represent the virus content. In the RPMI medium, WB and LDB were added to a final concentration of 0.25 × 10 ~ 5sfu of CFHIV. The concentration of AA in RPMI (4 parts) was 62.5, 125, 250 and 500μg / ml respectively, and the concentration of AA in WB and LDB was 500μg / ml. After the mixture was incubated for 3 hours at 4C, RPMI and WB were added