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以10个大麦优良品种为实验材料,成熟胚为外植体,研究基因型、种子的不同切割方式、培养基、激素等对大麦成熟胚愈伤组织的诱导及植株再生的影响。结果表明,种子纵切后接种出愈率显著高于横切;改良MS培养基能提高出愈率;在愈伤组织诱导过程中,不同品种对激素2,4-D与Dicamba的反应表现不同;初代愈伤组织经过3次继代培养后会转变为两种类型的胚性愈伤组织;不同品种的植株再生在不同浓度有机添加物的分化培养基上表现不同;长时间的继代培养,一些品种在植株再生过程中出现一定数量的白化苗。供试材料均能进行愈伤组织诱导,但是只有部分品种能再生植株。本实验筛选出愈伤组织诱导频率和绿苗分化率均较高,适合于遗传转化的受体材料,如87-3175、87-0053、97-40109、7-6004及208813-509。
Ten barley cultivars were used as experimental materials and mature embryos were used as explants to study the effects of genotypes, seed cutting patterns, media and hormones on the induction of embryogenic callus and plant regeneration in barley. The results showed that the inoculation rate was significantly higher than that of transection after seed slitting; improved MS medium could increase the rate of callus induction; different callus responses to hormone 2,4-D and Dicamba during callus induction were different ; Primary callus after three subcultures will be transformed into two types of embryogenic callus; different varieties of plant regeneration in different concentrations of organic additives differentiated medium on the performance of different; long-term subculture Some species appeared a certain amount of albino seedlings during plant regeneration. All the tested materials could be induced by callus, but only some of the cultivars could regenerate the plants. This experiment screened callus induction frequency and green shoot differentiation rate are high, suitable for genetic transformation of receptor materials, such as 87-3175,87-0053,97-40109,7-6004 and 208813-509.