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建立高效液相色谱法测定金银花中芳樟醇乙酯的方法。金银花通过水蒸气蒸馏提取挥发油。采样后以高效液相色谱法测定,色谱柱:Hypersil ODS2 C_(18)(150mm×4.6mm,5μm);流动相:甲醇-水(65:35,V/V);流速:1.0mL/min;柱温:室温;检测波长:220nm。在10.4—104.0μg·mL~(-1)浓度范围内线性关系良好(r=0.9995),平均回收率为100.7%,RSD=1.58%(n=9)。该方法简便、快速、准确,能有效控制金银花中芳樟醇乙酸酯的含量。
To establish a HPLC method for the determination of linalool ethyl linalool. Honeysuckle extract volatile oil by steam distillation. After the sample was measured by high performance liquid chromatography, the chromatographic column was Hypersil ODS2 C18 (150 mm × 4.6 mm, 5 μm); the mobile phase was methanol-water (65:35, V / V); the flow rate was 1.0 mL / min ; Column temperature: room temperature; detection wavelength: 220nm. The linearity was good (r = 0.9995) with the average recovery of 100.7% and RSD 1.58% (n = 9) in the range of 10.4-104.0μg · mL -1. The method is simple, rapid and accurate, and can effectively control the content of linalool acetate in honeysuckle.