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目的 :探讨星形细胞在慢性脑灌注不足脑损害中的作用。方法 :70只老龄大鼠持久性双侧颈总动脉结扎 (2VO) ,其中 12只接受环孢霉素A(CsA)胃灌治疗。免疫组化法多克隆抗血清GFAP标记星形细胞。实验研究为持久性 2VO 1~ 4月。结果 :持久性 2VO 1月皮层、海马和白质处的星形细胞明显增生、肥大。 1~ 4月 ,皮层、海马和白质处星形细胞继续增生、肥大 ,同时脑损害加重。CsA治疗后脑损害明显减轻 ,星形细胞的活动明显减少。结论 :大鼠慢性脑灌注不足可诱导星形细胞持久性增生、肥大 ,反应性星形细胞扮演神经保护作用。CsA可通过减轻脑损害 ,从而抑制大鼠慢性脑灌注不足脑内星形细胞的活动。
Objective: To investigate the role of astrocytes in brain damage caused by chronic cerebral hypoperfusion. Methods: Totally 70 aged rats were subjected to permanent bilateral common carotid artery ligation (2VO). Twelve of them received cyclosporine cyclosporine A (CsA) gastric perfusion. Immunohistochemistry Polyclonal antiserum GFAP - labeled astrocytes. Experimental study for the persistence of 2VO 1 ~ April. Results: The persistence of 2VO in January cortex, hippocampus and white matter at the astrocytes significantly hyperplasia, hypertrophy. From January to April, cortical, hippocampal and white matter astrocytes continue to proliferate, hypertrophy, and aggravate brain damage. CsA treatment significantly reduced brain damage, astrocyte activity was significantly reduced. CONCLUSION: Chronic cerebral hypoperfusion can induce persistent proliferation of astrocytes in rats, and hypertrophy and reactive astrocytes play a neuroprotective role. CsA can inhibit brain astrocyte activity in rats with chronic cerebral hypoperfusion by reducing brain damage.