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目的:研究芦荟大黄素对人胃癌细胞株SGC-7901的增殖抑制机制。方法:采用MTT方法观察芦荟大黄素对人胃癌细胞株SGC-7901的增殖抑制作用;采用流式细胞仪技术检测细胞周期和DNA含量;应用免疫组化方法观察PCNA的表达。结果:芦荟大黄素能抑制人胃癌细胞株SGC-7901的增殖(P<0.05);使G_0/G_1期细胞增多,使S期和G_2/M期的细胞明显减少,细胞增殖抑制率明显增高,DNA指数明显降低(P<0.05);芦荟大黄素能抑制PCNA的表达(P<0.05)。结论:芦荟大黄素抑制人胃癌细胞株SGC-7901的增殖,可能是通过抑制PCNA的表达而影响细胞周期的结果。
Objective: To study the inhibitory mechanism of aloe-emodin on the proliferation of human gastric cancer cell line SGC-7901. Methods: MTT assay was used to observe the inhibitory effect of aloe-emodin on the proliferation of human gastric cancer cell line SGC-7901. The cell cycle and DNA content were detected by flow cytometry. The expression of PCNA was detected by immunohistochemistry. Results: Aloe-emodin could inhibit the proliferation of human gastric cancer cell line SGC-7901 (P <0.05). Increasing the number of cells in G 0 / G 1 phase decreased the number of S phase and G 2 / M phase cells significantly, DNA index was significantly lower (P <0.05); aloe-emodin could inhibit the expression of PCNA (P <0.05). CONCLUSION: Aloe-emodin can inhibit the proliferation of human gastric cancer cell line SGC-7901 and may affect the cell cycle by inhibiting the expression of PCNA.