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目的探讨幽门螺杆菌感染与胃癌局部浸润之间的关系。方法收集我院2009年10月至2011年10月就诊的原发性胃癌患者共98例,获取患者的原发性胃癌标本,随机选取40例原发性胃癌手术切缘的正常组织作为对照。所有切片均作常规HE染色,用SP法进行免疫组化染色。用革兰染色法挑选出幽门螺杆菌阳性切片,对胃癌组切片其进行MMP-2和VEGF蛋白检测。结果 98例胃癌组织中幽门螺杆菌阳性者有68例,占69.4%。40例原发性胃癌患者的正常组织中幽门螺杆菌阳性者有7例,占17.5%。胃癌组中幽门螺杆菌阳性者MMP-2和VEGF蛋白表达阳性率明显高于幽门螺杆菌阴性者。结论幽门螺杆菌感染与胃癌局部浸润密切相关,并在胃癌的转移和浸润中作用突出。
Objective To investigate the relationship between Helicobacter pylori infection and local invasion of gastric cancer. Methods A total of 98 cases of primary gastric cancer were collected from October 2009 to October 2011 in our hospital. Primary gastric cancer specimens were obtained and 40 cases of primary gastric cancer resected margin were selected randomly as control. All sections were routine HE staining, SP immunohistochemical staining. Helicobacter pylori-positive sections were selected by Gram staining, and MMP-2 and VEGF protein were detected in gastric cancer sections. Results There were 68 cases of Helicobacter pylori positive in 98 cases of gastric cancer, accounting for 69.4%. Forty cases of patients with primary gastric cancer in the normal tissue Helicobacter pylori positive in 7 cases, accounting for 17.5%. The positive rate of MMP-2 and VEGF protein expression in Helicobacter pylori-positive gastric cancer group was significantly higher than Helicobacter pylori negative. Conclusion Helicobacter pylori infection is closely related to the local invasion of gastric cancer, and plays an important role in the metastasis and infiltration of gastric cancer.