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目的 初步了解STK11/LKB1的转录起始位点信息 ,分析其可能的候选启动子区段 ,为以后进行STK11/LKB1的转录调控研究作准备。方法 通过检索网上数据库资料 ,获得STK11/LKB1的转录本信息 ;运用MethPrimer和FirstEF两种程序 ,对包含STK11/LKB1基因“第一外显子”、“第一内含子”及其 5’UTR区上游的gDNA片段序列 ,分别进行CpG岛分析和第一外显子预测 ;利用PromoterInspector等预测程序 ,对STK11/LKB1的启动子进行预测。结果 目前已知的STK11/LKB1转录本中 ,5’UTR区最长的序列为BC0 0 7981(GenBank登录号 ) ;STK11/LKB1基因属于典型的CpG岛相关基因 ,现有的“第一外显子”之 5’上游已不再有外显子 ,已有的 5’UTR区基本已基本接近STK11/LKB1的转录起始位点 ;在BC0 0 7981的 5’上游约 2 0 0bp到 40 0bp内很可能存在有启动子。结论 STK11/LKB1已有的 5’UTR区基本已基本接近STK11/LKB1的转录起始位点 ,在BC0 0 7981的 5’上游数百bp内很可能能找到具有启动活性的区段从而有利于进行下一步的转录调控研究。实验明确其真实的转录起始位点应已相对容易。
OBJECTIVE: To understand the transcriptional start site of STK11 / LKB1 and to analyze its possible candidate promoter regions for future studies on the transcriptional regulation of STK11 / LKB1. Methods The transcript information of STK11 / LKB1 was obtained by searching the online database. Using MethPrimer and FirstEF programs, the expression of STK11 / LKB1 gene including “first exon”, “first intron” and its 5’UTR GDNA fragment sequence upstream of the region, respectively CpG island analysis and the first exon prediction; PromoterInspector other prediction program, the STK11 / LKB1 promoter prediction. Results The most known sequence of STK11 / LKB1 in the 5’UTR was BC0 0 7981 (GenBank accession number). The STK11 / LKB1 gene belongs to a typical CpG island related gene. The existing " There is no longer any exon in the upstream of 5 ’, the existing 5’ UTR region has basically been close to the transcription start site of STK11 / LKB1, and about 200 bp to 40 0 bp upstream of 5 ’of BC0 09898 Probably there is a promoter within. Conclusion The existing 5’UTR region of STK11 / LKB1 is basically close to the transcriptional start site of STK11 / LKB1. Within the region of hundreds of bp upstream of 5’UTR of BC0 09898, it is likely to find a promoter active region to facilitate Carry on the next transcription regulation research. It has been relatively easy to experimentally identify its true transcriptional start site.