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目的:探讨黄芪对感染血吸虫小鼠肝组织外周型苯二氮受体表达的影响。方法:采用黄芪注射液腹腔注射,在小鼠感染血吸虫满8周开始治疗,疗程12周,与生理盐水作对照。应用实时监测荧光定量PCR技术,于感染满8周、12周和20周时检测肝组织外周型苯二氮革受体mRNA的表达水平。结果:黄芪治疗组小鼠外周型苯二氮受体mRNA水平在感染12周和20周时均明显低于同期对照组,与正常组接近;黄芪治疗组治前、治中和治后外周型苯二氮受体mRNA水平比较差异无统计学意义,而对照组治疗前后差异显著。结论:黄芪可降低血吸虫病小鼠肝组织外周型苯二氮革受体mRNA表达水平,提示黄芪可能通过此机制阻止血吸虫病肝损伤的发展。
Objective: To investigate the effect of Astragalus on the expression of peripheral benzodiazepine receptor in liver tissue of mice infected with Schistosoma japonicum. METHODS: Intraperitoneal injection of Astragalus injection was used to start the treatment in mice infected with schistosomiasis for 8 weeks. The treatment course was 12 weeks and compared with saline. Real-time monitoring of real-time fluorescence quantitative PCR was used to detect the expression level of peripheral benzodiazepine receptor mRNA in liver tissue at 8 weeks, 12 weeks and 20 weeks after infection. Results: The mRNA levels of peripheral benzodiazepine receptors in mice treated with Astragalus membranaceus were significantly lower than those in the control group at 12 and 20 weeks of infection, and were similar to those in the normal group. Peripheral benzene in the Astragalus treatment group before, during, and after treatment was observed. There was no statistically significant difference in diazoxide receptor mRNA levels, but there was a significant difference between the control group and before and after treatment. Conclusion: Astragalus membranaceus can reduce the expression of peripheral benzodiazepine receptor mRNA in liver tissue of schistosomiasis mice, suggesting that Huangqi may prevent the development of hepatic injury of schistosomiasis through this mechanism.