论文部分内容阅读
目的:探讨非甾体类抗炎药阿司匹林对存在有β-连环素异常表达的T淋巴细胞白血病Molt-4细胞株的作用及机制。方法:MTT法观察阿司匹林对Molt-4细胞的增殖抑制作用,流式细胞仪(FCM)检测阿司匹林对Molt-4细胞细胞周期分布的影响。实时荧光定量RT-PCR法分析经不同浓度的阿司匹林处理后的Molt-4细胞β-连环素及cyclin D1基因的表达水平的影响。结果:阿司匹林呈剂量依赖性抑制Molt-4细胞的增殖,72h半数抑制浓度为1.58mmol/L;随浓度增加,cyclin D1基因及蛋白的表达水平均下调。结论:非甾体类抗炎药阿司匹林可能通过影响Wnt信号通路调节某些细胞周期相关基因的表达,将Molt-4细胞阻滞于G0/G1期,从而抑制白血病细胞株Molt-4的增殖。
Objective: To investigate the effect and mechanism of aspirin, a non-steroidal anti-inflammatory drug, on Molt-4 cell line with abnormal expression of β-catenin in T lymphoblastic leukemia cell line. Methods: The inhibitory effect of aspirin on the proliferation of Molt-4 cells was observed by MTT assay. The effect of aspirin on the cell cycle distribution of Molt-4 cells was analyzed by flow cytometry (FCM). Real-time fluorescent quantitative RT-PCR was used to analyze the expression of β-catenin and cyclin D1 gene in Molt-4 cells treated with different concentrations of aspirin. Results: Aspirin inhibited the proliferation of Molt-4 cells in a dose-dependent manner. The half-inhibitory concentration of 72 h was 1.58 mmol / L. The expression of cyclin D1 gene and protein was down-regulated with increasing concentration. CONCLUSIONS: Aspirin, a non-steroidal anti-inflammatory drug, may inhibit the proliferation of Molt-4 cells by affecting the Wnt signaling pathway and regulating the expression of certain cell cycle-related genes in the G0 / G1 phase of Molt-4 cells.