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目的探讨建立理想的创伤失血性休克模型的方法。方法 40只健康SD大鼠随机分为对照组和休克组。休克组在完成股动脉、股静脉插管后用电刀在腹正中切开约5 cm长的切口,通过动脉放血,在10 min内将血压维持在休克水平[平均动脉血压(MAP)降到40 mm Hg],约1 h后进行液体复苏0.5 h,而对照组在同等条件下不进行创伤和失血实验,而是观察1.5 h。实验结束后两组大鼠均拔出留置针,结扎血管并缝合切口,给予室温25℃保温、饲养。72 h后处死大鼠,检测大鼠肺、肠组织的水含量,用RT-PCR法检测肺、肠组织细胞间黏附分子-1(ICAM-1)的mRNA表达量,并做病理切片。数据采用SPSS 10.0软件处理,两组间死亡率的比较采用χ2检验,而两组间的肺、肠组织水含量及ICAM-1 mRNA表达量比较采用t检验,P<0.05为差异有统计学意义。结果休克组与对照组比较,肺、肠组织水含量[肺:(0.808±0.020)vs(0.557±0.024);肠:(0.748±0.028)vs(0.503±0.026)]、肺、肠组织ICAM-1 mRNA与-βactin的电泳灰度比值差异均有统计学意义(P均<0.05)。结论大鼠创伤失血性休克模型出现了类似临床上创伤失血性休克后的肺、肠组织损伤的表现,并有一定的死亡率。
Objective To explore a method to establish an ideal traumatic hemorrhagic shock model. Methods Forty healthy SD rats were randomly divided into control group and shock group. In the shock group, the femoral artery and femoral vein were cannulated with an electric knife to incise about 5 cm incision in the middle of the abdomen. The blood pressure was maintained at shock level within 10 min by arterial bleeding [mean arterial pressure (MAP) 40 mm Hg]. After about 1 h, liquid resuscitation was performed for 0.5 h, while in the control group, traumatic and blood loss experiments were not performed under the same conditions but for 1.5 h. After the experiment, both groups of rats were pulled out indwelling needle, ligation of blood vessels and suture incision, given room temperature 25 ℃ heat, feeding. After 72 h, the rats were sacrificed, and the water content in the lung and intestine of rats was measured. The mRNA expression of ICAM-1 in lung and intestine was detected by RT-PCR, and the pathological sections were made. The data were analyzed by SPSS 10.0 software, the mortality of the two groups was compared by χ2 test, while the water content of lung and intestine and the expression of ICAM-1 mRNA in the two groups were compared by t test, P <0.05 was considered statistically significant . Results Compared with the control group, the water content in the lung and intestine tissue in the shock group (lung: (0.808 ± 0.020) vs (0.557 ± 0.024) and (0.748 ± 0.028) vs (0.503 ± 0.026) 1 mRNA and -βactin electrophoresis grayscale differences were statistically significant (P all <0.05). Conclusion The model of traumatic hemorrhagic shock in rats shows the similar injury of lung and intestine after traumatic hemorrhagic shock in clinic, and has a certain mortality rate.