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以 2月龄黑松 (Pinusthunbergii)、湿地松 (P .elliottii)幼苗为材料 ,研究松材线虫(Bursaphelenchusxylophilus)感染对松幼苗活性氧代谢的影响。在接种松材线虫后 ,黑松较湿地松发病快且重 ,黑松内超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)活性逐渐降低 ;湿地松内SOD和CAT在接种早期活性增加 ,随后下降。两种幼苗接种后 ,过氧化物酶 (POD)活性、H2 O2 和丙二醛 (MDA)含量不断增加。H2 O2 和MDA的积累与植株发病速度呈明显正相关。因此 ,松材线虫病的发生和发展与寄主植物组织的膜脂过氧化作用有关
The effects of Bursaphelenchus xylophilus infection on active oxygen metabolism in Pinus thunbergii seedlings were studied using two-month-old Pinus thunbergii and P. elliottii seedlings. After the pine wood nematode was inoculated, the incidence of P. sibirica was faster and heavier than that of P. xylophilus, and the activities of superoxide dismutase (SOD) and catalase (CAT) in P. tabulaeformis decreased gradually; Increase, then decline. Peroxidase (POD) activity, H2O2, and malondialdehyde (MDA) levels increased with both seedlings inoculated. The accumulation of H2O2 and MDA was positively correlated with the disease incidence rate. Therefore, the occurrence and development of pine wilt disease and the host plant membrane lipid peroxidation