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目的建立呼吸道合胞病毒(RSV)致病模型,评价麻杏石甘汤对该病的疗效。方法 SD大鼠被随机分成5组:对照组、RSV病毒感染模型组和麻杏石甘汤组高、中、低剂量组,每组6只。除对照组外,其它组经RSV滴鼻吸入感染1天,建立病毒感染模型。第2天起,麻杏石甘汤高、中、低剂量组分别按以临床剂量6.3,4.2,2.8g/kg给予麻杏石甘汤水溶液灌胃,正常组、模型组给予等剂量的生理盐水灌胃,一天1次,连续5天。气管插管后使用肺功能检测仪检测肺功能。HE染色后在光镜下观察各组大鼠肺组织炎症变化情况。结果 1与对照组比,模型组湿干比显著增加(P<0.01);肺部病理损害明显;肺功能指标改变明显,模型组TI、TE、EIP、EEP、EV、PAU、TV和MV值降低,RT、PEF、PENH和PIF升高,差异有统计学意义(P<0.05或P<0.01)。2与模型组相比,麻杏石甘汤治疗组大鼠一般状态、肺功能及肺组织形态学指标均有改善。麻杏石甘汤低剂量组大鼠TE、EEP、TI和PAU升高,PEF、PIF、PENH和RT降低,与模型组相比有显著性差异(P<0.05或P<0.01);麻杏石甘汤中剂量组大鼠TE、EIP、EEP、EV、TI和TV升高,PIF、PEF、PENH和RT降低,与模型组相比有显著性差异(P<0.05或P<0.01);麻杏石甘汤高剂量组大鼠TE、EEP、PEF、EV和MV升高,PENH降低,与模型组相比有显著性差异(P<0.05或P<0.01)。结论麻杏石甘汤能明显地改善RSV肺炎。
Objective To establish a pathogenic model of respiratory syncytial virus (RSV) and evaluate the therapeutic effect of Maxingshigan Decoction on this disease. Methods SD rats were randomly divided into five groups: control group, model group with RSV virus infection and high, medium and low dose groups of Maxing Shigan Decoction group, with 6 rats in each group. In addition to the control group, the other groups were infected by RSV nasal inhalation for 1 day to establish a virus infection model. On the second day, Maxing Shigan Decoction high, middle and low dose groups were given at the clinical dose of 6.3,4.2,2.8 g / kg Maxing Shigan Decoction gavage, normal group, model group were given equal doses of physiology Saline irrigation, 1 day, for 5 days. Pulmonary intubation pulmonary function test instrument to detect lung function. After HE staining, the changes of lung inflammation in each group were observed under light microscope. Results Compared with the control group, the wet-dry ratio of the model group was significantly increased (P <0.01); the pathological damage of lung was obvious; the indexes of pulmonary function changed obviously; the values of TI, TE, EIP, Decreased, RT, PEF, PENH and PIF increased, the difference was statistically significant (P <0.05 or P <0.01). Compared with the model group, the general state, lung function and lung histological parameters of Maxing Shigan Decoction group were improved. The contents of TE, EEP, TI and PAU, PEF, PIF, PENH and RT in low-dose Maxingshigan decoction group were significantly lower than those in model group (P <0.05 or P <0.01) The levels of TE, EIP, EEP, EV, TI and TV in the medium dose Shigan Decoction group were significantly lower than those in the model group (P <0.05 or P <0.01). Compared with the model group, the Maxing Shigan Decoction high dose group increased TE, EEP, PEF, EV and MV, PENH decreased significantly (P <0.05 or P <0.01). Conclusion Maxing Shigan Decoction can significantly improve RSV pneumonia.