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谷胱甘肽(GSH)是一种含有巯基的三肽分子,参与许多细胞内生化过程,具有抗氧化和整合解毒功能,在生物体内以及医学,食品等领域有着极为重要的作用。GSH参与细胞内、体液中的许多重要生化反应,其在人体内含量的变化,相应地提示了人体的健康问题。目前对GSH的检测手段有表面增强拉曼光谱(SERS)、电化学分析、高效液相色谱(HPLC)等,这些方法大都操作复杂、耗时较长或者需要昂贵的仪器。利用一种新型荧光银纳米团簇(Ag NCs)作为探针,通过同时分析银纳米团簇的荧光强度变化以及荧光峰位置移动实现了GSH的高精度快速检测。在检测过程中,GSH分子与荧光探针发生化学反应,改变了荧光探针的光化学特性,其荧光强度因发生猝灭而减弱,且其荧光峰位置因配体的改变也发生移动。通过对照组实验,我们进一步证明了所发展的检测方法对GSH目标具有很好的特异性,综合考察荧光强度和波长的变化数据可以很好地区分GSH以及其他结构类似的分子,同时探针对于多种盐离子及氨基酸等不敏感,能够很好地保证检测的准确性。我们报导的荧光探针合成步骤简单,过程绿色环保,GSH检测的响应速度快、光谱波动较小、相对误差小。进一步的研究有望实现细胞内的GSH高精度检测及成像。
Glutathione (GSH), a thiol-containing tripeptide molecule involved in many intracellular biochemical processes, has antioxidant and detoxification functions and plays an extremely important role in the body and in medicine and foodstuffs. GSH is involved in many important biochemical reactions in the cells and body fluids, and its content in the human body changes accordingly, suggesting the health problems of the human body. At present, there are surface enhanced Raman spectroscopy (SERS), electrochemical analysis and high performance liquid chromatography (HPLC) for detecting GSH. Most of these methods are complicated, time-consuming or require expensive instruments. A new type of fluorescent silver nanoclusters (Ag NCs) was used as a probe to rapidly and accurately detect GSH by simultaneously analyzing the fluorescence intensity changes of silver nanoclusters and the position of the fluorescence peak. During the detection process, GSH molecules react with the fluorescent probe to change the photochemical properties of the fluorescent probe. The fluorescence intensity of the fluorescent probe is weakened due to quenching, and the position of the fluorescence peak also shifts due to the change of the ligand. Through the control group experiments, we further proved that the developed detection method has a good specificity for the GSH target. Comprehensively examining the fluorescence intensity and wavelength variation data can well distinguish between GSH and other structurally similar molecules. At the same time, A variety of salt ions and amino acids are not sensitive, can well guarantee the accuracy of detection. We reported a simple fluorescent probe synthesis process, the process of green, GSH detection of fast response, small spectral fluctuations, the relative error is small. Further research is expected to achieve high-precision intracellular GSH detection and imaging.