目的:探索益气利水破血解毒小复方干预对NZB/W F1狼疮鼠肾脏水通道蛋白AQP1和AQP3表达的影响,帮助进一步了解其作用机制和靶点。方法:将NZB/W F1狼疮鼠随机分为对照组(0.5ml/d生理盐水灌胃)、中药治疗组(益气利水破血解毒小复方0.5ml/d灌胃)和西药治疗组(骁悉水溶液0.5ml/d灌胃),分别于干预第0、2、4、6周留取尿液样本检测尿蛋白水平,干预8周后处死小鼠行肾脏免疫组化,半定量分析AQPl、AQP3表达水平。结果:中药组和西药组小鼠尿蛋白定量均明显低于对照组(P<0.01),两治疗组间未见统计差异(P>0.05);中药组和西药组小鼠肾组织AQP1表达量显著高于对照组(P<0.01),西药组AQP1表达量高于中药二组间存在统计学差异(p<0.05)。与对照组相比,中药组和西药组小鼠肾组织AQP3表达量显著较高,有统计学差异(p<0.01),西药组与中药组小鼠肾组织AQP3表达量未见统计学差异(p>0.05)。结论:益气利水破血解毒小复方干预可有效降低NZB/WF1狼疮鼠尿蛋白水平,并加强肾脏水通道蛋白AQP1和AQP3的表达。 OBJECTIVE: To explore the effect of Yiqilizhui, Huoxue detoxification small interfering on renal AQP1 and AQP3 expression in NZB / W F1 wolf lupus mice to help further understand its mechanism of action and target. Methods: The NZB / W F1 lupus mice were randomly divided into control group (0.5ml / d saline), Chinese medicine group (0.5ml / d gavage Yiqili Shuijiedujiedu) and western medicine group Aqueous extract of 0.5ml / d gavage), urine samples were taken at the 0, 2, 4 and 6 weeks of intervention to detect urinary protein levels. After 8 weeks of intervention, mice were sacrificed and kidneys were immunohistochemically stained. Semiquantitative analysis of AQP1, AQP3 expression level. Results: The urinary protein in the two groups was significantly lower than that in the control group (P <0.01), but there was no statistical difference between the two groups (P> 0.05). The expression of AQP1 in the kidney of the Chinese medicine group and the western medicine group (P <0.01). The expression of AQP1 in western medicine group was higher than that in traditional Chinese medicine group (p <0.05). Compared with the control group, the expression of AQP3 in the kidney of Chinese medicine group and western medicine group was significantly higher (p <0.01), and there was no significant difference in the expression of AQP3 in the kidney of Western medicine group and traditional Chinese medicine group p> 0.05). Conclusion: The intervention of Yiqi Lishui blood detoxification small dose can effectively reduce the urinary protein level of NZB / WF1 lupus mice and enhance the expression of AQP1 and AQP3 in kidney.