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目的:探讨不同干预状态下大鼠结肠主要运动模式——长距离收缩(LDC)的特征。方法:取40只雄性SD大鼠随机分为3组,分别给予酵母抽提物(YE)及生理盐水(对照组)急性灌胃3d、神经阻断剂河豚毒素(TTX)和氯贝胆碱(bethanechol)干预、将结肠均分为近远端独立观察,用时空图记录大鼠结肠的基本运动模式LDC的变化。结果:营养物质酵母抽提物(YE)可使LDC收缩时间延长,传播速度减慢;胆碱能M1受体激动剂氯贝胆碱可在神经阻断剂河豚毒素(TTX)作用的情况下使结肠重新出现LDC,但收缩速度同样减慢,变为巨大而缓慢推进的LDC;将结肠头尾端均分开后,尾端结肠仍可独立于近端结肠而出现LDC,其收缩速度明显快于近端结肠,传播时间缩短。结论:营养物质、药物等都可使LDC出现不同的特征性改变,这些运动模式的变化主要体现了结肠的肌源性活性及自主起搏功能,从而对消化吸收产生综合调节作用。
OBJECTIVE: To investigate the main colon movement pattern of rats with long-term contractions (LDC) under different interventions. Methods: Forty male Sprague-Dawley rats were randomly divided into three groups. The animals were treated with YE and saline (control group) for 3 days, and the neurotransmitter tetrodotoxin (TTX) (bethanechol) intervention, the colon were divided into near and far independent observation, the spatial and temporal maps recorded the changes in rat’s basic pattern of exercise LDC. Results: Nutrient yeast extract (YE) prolonged the contractile time of LDC and slowed down the transmission. Cholinergic cholinergic M1 receptor agonist, cholinergic antagonist, could block the action of tetrodotoxin (TTX) LDC re-emergence of the colon, but the same slowed down the rate of contraction into a huge and slowly advancing LDC; will be separated after the head and tail of the colon, the tail colon can still appear independent of the proximal colon LDC, the contraction rate was significantly faster In the proximal colon, transmission time is shortened. Conclusion: Nutrients and drugs can make different characteristic changes of LDC. The changes of these exercise modes mainly reflect the myogenic activity of the colon and autonomic pacing function, so as to have a comprehensive regulation of digestion and absorption.