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目的：探讨肿瘤转移抑制相关基因nm23－H1，转移相关基因CD44v6在甲状腺乳头状癌的表达与颈淋巴结转移的关系。方法：采用免疫组化S－P法，检测201例甲状腺乳头状癌、30例甲状腺良性病变及30例正常甲状腺组织中nm23－H1和CD44v6的表达情况。结果：1）nm23－H1在甲状腺乳头状癌组的表达阳性率为62．7％，明显低于良性病变组和正常组（P＜0．01或P＜0．05）。有转移组nm23－H1的阳性率为54．5％，明显低于无转移组（75．0％），其蛋白表达与颈淋巴结转移呈负相关。2）CD44v6在甲状腺乳头状癌组的表达阳性率为71．1％，明显高于两对照组（P均＜0．01）。有转移组CD44v6的阳性率为79．3％，明显高于无转移组（58．5％），其蛋白表达与颈淋巴结转移呈正相关。3）甲状腺乳头状癌中nm23－H1和CD44v6表达呈显著负相关关系（P＜0．01）。nm23－H1阴性表达伴CD44v6阳性表达的患者发生颈淋巴结转移的可能性大。结论：nm23－H1和CD44v6的表达与甲状腺乳头状癌的颈淋巴结转移密切相关，nm23－H1和CD44v6的表达在淋巴结转移中起协同作用，两者的表达失衡可能与甲状腺乳头状癌发生颈淋巴结转移有密切关系。因此，检测nm23－H1和CD44v6可以作为预测甲状腺乳头状癌颈淋巴结转移潜能的有价值的参考指标。
Objective: To investigate the relationship between tumor metastasis suppressor gene nm23-H1 and metastasis-related gene CD44v6 in thyroid papillary carcinoma and cervical lymph node metastasis. METHODS: Immunohistochemical S-P method was used to detect the expression of nm23-H1 and CD44v6 in 201 cases of papillary thyroid carcinoma, 30 cases of thyroid benign lesions and 30 cases of normal thyroid tissues. Results: 1) The positive expression rate of nm23-H1 in papillary thyroid carcinoma group was 62.7%, which was significantly lower than that of benign lesion group and normal group (P<0.01 or P<0.05). The positive rate of nm23-H1 in the metastatic group was 54.5%, which was significantly lower than that in the non-metastatic group (75.0%). The protein expression was negatively correlated with cervical lymph node metastasis. 2) The positive expression rate of CD44v6 in papillary thyroid carcinoma was 71.1%, which was significantly higher than that of the two control groups (P <0.01). The positive rate of CD44v6 in the metastatic group was 79.3%, which was significantly higher than that in the non-metastasis group (58.5%). The protein expression was positively correlated with cervical lymph node metastasis. 3) There was a significant negative correlation between the expression of nm23-H1 and CD44v6 in papillary thyroid carcinoma (P<0.01). Patients with negative expression of nm23-H1 with positive expression of CD44v6 are more likely to have cervical lymph node metastasis. Conclusion: The expression of nm23-H1 and CD44v6 is closely related to the cervical lymph node metastasis of papillary thyroid carcinoma. The expression of nm23-H1 and CD44v6 play a synergistic role in lymph node metastasis. The imbalance of the expression of nm23-H1 and CD44v6 may be associated with the occurrence of cervical lymph node in papillary thyroid carcinoma. Transfers are closely related. Therefore, the detection of nm23-H1 and CD44v6 may serve as a valuable reference for predicting the metastatic potential of cervical lymph nodes in papillary thyroid carcinoma.