论文部分内容阅读
本实验应用形态测量技术观察了大鼠冲击伤时肺泡上皮细胞和毛细血管内皮细胞紧密连接冷冻蚀刻形态学变化。实验分四组进行。Ⅰ组为未致伤的正常对照组,ⅡⅢⅣ组分别于伤后4—6小时,24小时和72小时活杀。以模拟激波管致伤。致伤组动物总体为中等度以上损伤。以听器及肺损伤最明显。肺损伤主要表现为肺出血。Ⅱ组Ⅲ组动物有肺水肿,Ⅳ组动物肺水肿已吸收。一、冷冻蚀刻一般观察本实验共观察了38只大鼠的冷冻蚀刻电镜。共观察到可用于统计的肺泡上皮细胞TJ235个,血管内皮细胞TJ207个。另观察到5个肺泡上皮细胞TJ“残迹”和一个毛细血管内皮细胞TJ
In this experiment, Morphometry was used to observe the morphological changes of the tight junctions of alveolar epithelial cells and capillary endothelial cells during shock injury in rats. The experiment was divided into four groups. Group Ⅰ was not injured normal control group, Ⅱ Ⅲ Ⅳ group were killed 4-6 hours after injury, 24 hours and 72 hours live kill. To simulate shock tube injury. The animals in the injury group were moderately above injury. Listener and lung injury most obvious. Lung injury is mainly manifested as pulmonary hemorrhage. Ⅱ group Ⅲ animals have pulmonary edema, Ⅳ group had pulmonary edema has been absorbed. First, the general observation of frozen etching observed in this experiment a total of 38 rats by freeze-etching electron microscopy. A total of TJ235 alveolar epithelial cells and TJ207 vascular endothelial cells were observed. Another observed five alveolar epithelial TJ “remnants of” and a capillary endothelial cell TJ