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SQUAMOSA promoter-binding-like(SPL)基因家族作为一个植物特异转录因子家族,已被证实具有许多重要的生物学功能。本研究利用19条水稻SPL基因序列设计的引物,对引自美国农业部的171份水稻微核心种质的Oryza sativaL.SQUAMOSA promoter-binding-like(OsSPL)基因家族进行多态性分析。19对OsSPL基因引物扩增出140个条带,其中128个条带具有多态性,占91.4%;平均每个位点检测到7.4个条带。聚类与主坐标分析将171份材料划分为6类,类内品系呈相似地理生态分布,类与类之间无亚洲栽培稻与非洲栽培稻分化。性状关联分析表明,OsSPL基因家族的基因与每穗粒数、株高和抽穗期相关联。
As a family of plant-specific transcription factors, the SQUAMOSA promoter-binding-like (SPL) gene family has been identified as having many important biological functions. In this study, polymorphisms of 171 Oryza sativa L.SQUAMOSA promoter-binding-like (OsSPL) gene families derived from the USDA microalgal core germplasm were analyzed using 19 primers designed from the SPL gene sequences of rice. 19 pairs of OsSPL gene primers amplified 140 bands, of which 128 bands were polymorphic, accounting for 91.4%; an average of 7.4 bands per locus. Cluster analysis and principal coordinate analysis divided 171 materials into 6 categories with similar geographical and ecological distributions. There was no differentiation between Asian cultivated rice and African cultivated rice in the class. Trait correlation analysis showed that OsSPL gene family genes were associated with grain number per spike, plant height and heading date.